Extended Data Fig. 5. Properties of neuroblastoma immunopeptidome.

a. Logo plots of peptides eluted from HLA-A2 and HLA-A24 show canonical binding motifs for both alleles⁶⁴. b. Peptides detected by immunopeptidomics are enriched for highly expressed parent genes as compared to the entire cellular transcriptome (p = 2.2 × 10⁻¹⁶ – 0.00059 across cell lines). c. Gene ontology analysis of the parent genes of peptides of the neuroblastoma immunopeptidome compared to the cellular transcriptome. The most significantly enriched ontology groups are nucleic acid binding proteins (RNA binding protein ontology p = 1.22e⁻²⁵ and nucleic acid binding ontology p = 9.47e⁻¹⁷; Gene Ontology analyses were performed using PANTHER⁴¹ (http://geneontology.org/) and p-values calculated using Fischer’s Exact test).