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Construction of the pKSΔ6-1 Rhodococcus-E. coli shuttle vector for sacB expression in Rhodococcus strain 10-2. MCS, multiple-cloning site (expanded region). Blunt-end cloning of the end-filled 3.8-kb BamHI fragment of pUM28 plasmid containing sacB (33) at the EcoRV site is indicated.
