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. 2023 Nov 22;13:20477. doi: 10.1038/s41598-023-46852-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pro-inflammatory stimulation on Q22 and Q109 iPSC-microglia. (a) iPSC-microglia were stained for phalloidin and morphology was assessed. Both Q109 and Q22 pro-inflammatory stimulated iPSC-microglia exhibited a significantly increased cell area and decreased roundness compared to unstimulated cells. No differences were detected between Q109 and Q22 iPSC-microglia. (b) Pro-inflammatory stimulation increased secretion of IL-8 significantly, and IL-6 in both Q109 and Q22 iPSC-microglia. (c) Phagocytosis of E. coli particles was significantly reduced after pro-inflammatory stimulation in both Q109 and Q22 iPSC-microglia. The significant difference in phagocytosis capacity in unstimulated iPSC-microglia disappeared after pro-inflammatory stimulation. (d) Endocytosis of transferrin is significantly reduced after pro-inflammatory stimulation in both, Q109 and Q22 iPSC-microglia. The significant difference in endocytosis capacity in unstimulated iPSC-microglia disappeared after pro-inflammatory stimulation. Data are expressed as mean ± SEM. n = 3 independent experimental repeats with 3 clones. Statistical analysis was performed using two-way ANOVA and Tukey’s honest significance test. *p < 0.05; **p < 0.01; ***p < 0.001.