Table 1.
Representation of C. rhombifer eDNA sequence detection at each surveyed locality in each technical and biological replicate sampled when using VertCOI primers and after data analysis in mBRAVE. The Table did not include localities where no C. rhombifer eDNA detection was found in any replicate.
| Locality | Tech replicate | 1st biological replicate | 2nd biological replicate | ||||
|---|---|---|---|---|---|---|---|
| Reads | ML | MS (%) | Reads | ML | MS (%) | ||
| Zanja del Diez | I | 16,060 | 184.95 | 99.89 | 9422 | 184.96 | 99.81 |
| II | 1 | 185 | 100 | 22,759 | 184.95 | 99.89 | |
| III | 2 | 185 | 100 | 3 | 185 | 100 | |
| Zanja del Nueve | I | – | – | – | 1 | 185 | 100 |
| II | 1 | 185 | 100 | 4 | 185 | 99.86 | |
| III | – | – | – | 2 | 185 | 100 | |
| Estamento | I | – | – | – | – | – | – |
| II | – | – | – | – | – | – | |
| III | – | – | – | 2 | 184.5 | 100 | |
| Punta Arena | I | – | – | – | – | – | – |
| II | – | – | – | – | – | – | |
| III | – | – | – | 1 | 185 | 100 | |
| Estero de Punta Arena | I | 2 | 185 | 100 | – | – | – |
| II | – | – | – | – | – | – | |
| III | 2 | 185 | 100 | – | – | – | |
| Laguna de Vitorino | I | 35,841 | 184.96 | 99.91 | 66,164 | 184.96 | 99.93 |
| II | 25,258 | 184.95 | 99.91 | 48,288 | 184.96 | 99.93 | |
| III | 37,499 | 184.95 | 99.92 | 91,116 | 184.95 | 99.93 | |
| Lagunitas | I | 4 | 185.25 | 99.73 | – | – | – |
| II | 10 | 185 | 99.89 | – | – | – | |
| III | 13 | 185 | 100 | – | – | – | |
| Zanja de Santo Tomás | I | 1 | 185 | 100 | – | – | – |
| II | 3 | 185 | 100 | – | – | – | |
| III | – | – | – | – | – | – | |
| Canal de los Patos I | I | 12 | 184.92 | 99.82 | 5 | 185 | 99.89 |
| II | – | – | – | 6 | 185 | 100 | |
| III | – | – | – | 5 | 185 | 99.78 | |
| Canal de los Patos II | I | 5 | 185 | 99.46 | – | – | – |
| II | 2 | 185 | 99.46 | 2 | 185 | 100 | |
| III | 6 | 185 | 99.91 | 1 | 185 | 100 | |
| Laguna Nueva | I | – | – | – | 10 | 185 | 99.89 |
| II | – | – | – | 31 | 184.77 | 99.86 | |
| III | – | – | – | 20 | 185 | 100 | |
Average sequence length (ML), average mean similarity (MS%) to the reference haplotypes, and the total number of reads (Reads) generated from each technical replicate are included.