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. 2023 Nov 22;14(11):759. doi: 10.1038/s41419-023-06280-3

Fig. 5. LncRNA FTX regulates FNE1 expression through TET2-mediated FEN1 promoter demethylation.

Fig. 5

A, B The effect of lncRNA FTX transfection on FEN1 expression detected by qRT-PCR (A) and western blotting (B). Student’s t test for two-group comparison: **P < 0.01. C Prediction of CpG island enrichment in FEN1 promoter region by the MethPrimer website. D, E The expression of FEN1 in WSU-HN6 and SCC15 cells treated with 5-Aza-dC detected by western blotting (D) and qRT-PCR (E). Student’s t test for two-group comparison: **P < 0.01. F RPIseq databank predicts DNA demethylase TET2 are relevant to FTX using RF classifier and SVM classifier (RF > 0.5 & SVM > 0.5). G Potential binding sites for FTX within FEN1 promoter region verified using BLAST. H The enrichment of TET2 in FEN1 promoter region assessed by CHIP assay, and quantified by qRT-PCR. Student’s t test for two-group comparison: *P < 0.05; **P < 0.01. I The binding relationship between FTX and TET2 examined by RNA pull-down. J The enrichment of FTX by TET2 assessed by RIP assay, and quantified by qRT-PCR. Student’s t test for two-group comparison: **P < 0.01.