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. 2023 Nov 22;14(11):759. doi: 10.1038/s41419-023-06280-3

Fig. 7. FEN1 expression is negatively correlated with ACSL4 expression in OSCC.

Fig. 7

A The expression of ferroptosis-related markers expression in FEN1-transfected OSCC cell lines detected by western blotting. B Representative immunohistochemical staining images of GPX4, SLC7A11, and ACSL4 in xenografts were shown. Scale bar, 100 μm. C The correlation between ACSL4 and FEN1 mRNA expression in fresh OSCC tissue samples (n = 32). D The expression of FEN1 and ACSL4 in OSCC cells transfected with FEN1 and ACSL4 plasmid was measured by western blotting. E, F Schematic view of the reporter constructs containing different intervals of the ACSL4 regulatory region, generated for the mapping of the FEN1 responsive region (E). Luciferase activity was measured 48 h post-transfection (F). one-way ANOVA for multi-group comparisons: **P < 0.01. G ChIP-qPCR assay of FEN1 and normal IgG in SCC15 and WSU-HN6 cells. Student’s t test for two-group comparison: **P < 0.01. H Luciferase assay of increasing amount of FEN1 on the luciferase activity of pGL3-Basic and pGL3-ACSL4 plasmid in 293 T cells. Student’s t test for two-group comparison: *P < 0.05; **P < 0.01. I Transwell assay was used to determine the effect of a FEN1 and ACSL4 plasmid transfection on migration and invasion of OSCC cells. Scale bar, 200 μm. one-way ANOVA for multi-group comparisons: *P < 0.05; **P < 0.01. J A proposed model illustrating the role of PDPN+ CAFs derived exosomal lncRNA FTX in regulating motility of OSCC cells by inhibiting ferroptosis through FTX/FEN1/ACSL4 signaling cascade.