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. 2023 Nov 22;14:7629. doi: 10.1038/s41467-023-43295-y

Fig. 3. GmSNAP02 gene expression in response to SCN infection.

Fig. 3

a Nematode development on Peking, PI 90763, and PI 437654 soybean lines. Roots of 3-day-old seedlings were inoculated with infective second-stage juveniles (J2) and stained with acid fuchsin at 3 and 5 days post inoculation (dpi). Representative images of 3–5 independent roots/genotype are shown. White arrowheads denote swollen parasitic juvenile nematodes in Peking indicative of successful feeding site establishment and development. Scale bar = 500 μm. b Relative gene expression analysis of GmSNAP02 in mock-inoculated and SCN-infected roots using qRT-PCR. Two residual heterozygote-derived lines near-isogenic for GmSNAP02, 19AS-84-5-81-4 and 19AS-84-5-81-8, were also included. Statistical analysis was performed by a two-tailed Student’s t test. Data are means ± s.e.m. of three technical replicates for each biological replicate. Data from three independent biological replicates showed a significant (*P < 0.05, **P < 0.01) increase in GmSNAP02 gene in expression in susceptible Peking and 81-4, but not PI 90763, 81-8, and PI 437654 upon SCN HG type 1.2.5.7 (Race 2) infection.