Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 22;14:7617. doi: 10.1038/s41467-023-41938-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Amino acid sequences of juxtamembrane (JM) region of BTN3A1, BTN3A2, BTN3A3, and alpaca BTN3 (Vp) were aligned, and KKK and ETE residues of BTN3A1 and BTN3A3 were marked in red and blue, respectively. b Total and surface-expressed FLAG protein of permeabilized and live 3KO cells transduced with FLAG V∆3A1 alone or cotransduced with 3A3 or 3A3_KKK mutant detected by anti-FLAG and anti-mouse F(ab’)2-APC conjugate were shown as histograms. c 3KO cells transduced with 3A1mC, 3A3_R381H-mC, or 3A3_R381H_KKK-mC mutant were cocultured with 53/4 Vγ9Vδ2 TCR reporter cells and titrated concentration of HMBPP. The activation of reporter cells was measured by mouse IL-2 ELISA (n-3). d Models of the BTN3-JM coiled-coil dimers. Models of the predicted JM coiled-coil dimers Q273–L312 were generated using CCBuilder2 (see “Methods”). Dimer interface residues at positions 283–285 are shown as ball and stick. (I) BTN3A3 coiled-coil homodimer, (II) BTN3A2 coiled-coil homodimer, (III) Alpaca BTN3 (VpBTN3) coiled-coil homodimer, (IV) BTN3A1 coiled-coil homodimer, (V) BTN3A1-BTN3A2 coiled-coil heterodimer, (VI) BTN3A1-BTN3A3 coiled-coil heterodimer, (VII) BTN3A3-KKK (replacing ETE with KKK at positions 283–285) coiled-coil homodimer. Polar interactions are highlighted (red dashed lines). Each monomer within the homodimer has been labeled A or B. Graphical data are presented as mean with SD and analyzed by ordinary two-way ANOVA and SD was shown as error bars.