Skip to main content
. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure 1. Base excision repair activity during myofibrillogenesis.

Figure 1.

(A, B) Recruitment curve of FEN1-YFP on the locally damaged DNA (LD) by laser-micro irradiation (A) in myoblasts (MB, blue curve) isolated from the Fen1-YFP mouse model) and 7 d differentiated myonuclei (MT, red curve) and (B) in primary dermal fibroblast (F) isolated from the Fen1-YFP mouse model (green curve) and the same fibroblasts fused with PEG (FF, orange curve). (A, B) Error bars represent the SEM obtained from at least 15 nuclei in (A) and 19 nuclei for F and 12 nuclei for FF cells in (B) from N ≥ 3 independent experiments. (C) Sequential images of FEN1-YFP recruitment onto LD by α-particle microbeam irradiation in MB (upper panel) isolated from the Fen1-YFP mouse model and in MT (lower panel). The damaged areas are underlined by a dotted cross within the nucleus. The scale bar represents 10 μm. (D, E) Recruitment curve of FEN1-YFP on the LD by α-particle microbeam irradiation (D) in MB (blue curve) and MT (red curve) and (E) in primary dermal fibroblast (F) isolated from the Fen1-YFP mouse model (green curve) and the same fibroblasts fused with PEG (FF, orange curve). The irradiation was applied at t = 10 s. (D, E) Error bars represent the SEM obtained from N ≥ 3 independent experiments with 42–46 nuclei/cell type in (D) and 37–47 nuclei/cell type in (E). (F) Sequential imaging of FEN1-YFP turnover on the LD by laser irradiation in MB (upper panel) and MT (lower panel). The damaged area is underlined by a dotted cross in the nucleus. Scale bar, 5 μm. (G) Turnover curve of FEN1-YFP on the LD by laser irradiation in MB (blue curve) and MT (red curve). Error bars represent the SEM obtained from N ≥ 3 independent experiment with 10 nuclei.