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. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure 2. Disappearance of double-strand break signaling upon X-ray irradiation.

Figure 2.

(A) Representative images of stably 53BP1-GFP (green) expressing C2C7 myogenic cells at different state of differentiation, myoblasts (MB, upper panel), myocytes (MC, middle panel), and myotubes (MT, lower panel) at the indicated time post-5 Gy of X-ray irradiation, immunolabelled with antibodies against the homologous recombination (HR) factor RAD51 (magenta), and a double strand break marker, the phosphorylated histone H2AX protein (γ-H2AX, red). DNA was stained with DAPI (grey). Scale bars, 10 μm. (B, C, D) Quantification of γH2AX foci (B), 53BP1 foci (C), and RAD51 foci (D) per nucleus in stably 53BP1-GFP expressing C2C7 MB (blue curve), MC (dashed black curve), and MT (red curve) upon 5 Gy of X-ray irradiation (time “0” corresponds foci numbers in nonirradiated cells). N ≥ 3 independent experiments with 35–135 nuclei/cell type. Mean ± SEM, significance by one-way ANOVA with post-hoc Tukey’s multiple comparison test against MB at each time point, significant P-value figures are the same colour as the condition compared with ns P > 0,05, *P < 0.05, **P < 0.01, ***P < 0.001.