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. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure 3. Activity of an early nonhomologous end joining protein during myofibrillogenesis.

Figure 3.

(A) Sequential imaging of KU80-GFP recruitment onto the locally damaged DNA (LD) by laser-micro irradiation in stably KU80-GFP expressing C2C7 myoblasts (MB, upper panel) and myotubes (MT, lower panel). The damaged area is underlined by a dotted cross in the nucleus. Scale bar, 5 μm. (B) Recruitment curve of KU80-GFP on the LD by laser irradiation in stably KU80-GFP expressing C2C7 MB (blue curve) and MT (red curve). Error bars represent the SEM obtained from N ≥ 3 independent experiment with 10 nuclei. (C) Sequential imaging of KU80-GFP recruitment onto the LD by α-particle microbeam irradiation in stably KU80-GFP expressing C2C7 MB (upper panel) and MT (lower panel). The damaged area is underlined by a dotted cross in the nucleus. Scale bar, 10 μm. (D) Recruitment curve of KU80-GFP on the LD by α-particle microbeam irradiation in stably KU80-GFP expressing C2C7 myoblasts and myocytes (MB + MC, blue curve) and MT (red curve). The irradiation was applied at t = 10 s. N ≥ 3 independent experiments with 143–360 nuclei/cell type, and mean ± SEM. (E) Sequential imaging of KU80-GFP turnover on the LD by laser irradiation in stably KU80-GFP expressing C2C7 MB (upper panel) and MT (lower panel). The damaged area is underlined by a dotted cross in the nucleus. Scale bar, 5 μm. (F) Turnover curve of KU80-GFP on the LD by laser irradiation in stably KU80-GFP expressing C2C7 MB (blue curve) and MT (red curve). Error bars represent the SEM obtained from N ≥ 3 independent experiment with 10 nuclei.