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. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure 5. 53BP1, a DNA damage response factor, has almost no recruitment in multinucleated myotubes to local DNA damage.

Figure 5.

(A) Sequential images of 53BP1-GFP recruitment to the locally damaged DNA (LD) by α-particle microbeam irradiation in stably 53BP1-GFP expressing C2C7 myoblasts (MB, upper panel), myocyte (MC, middle panel), and myotubes (MT, lower panel). The damaged areas are underlined by a dotted cross in the nucleus. Scale bar, 10 μm. (B) Recruitment curve of 53BP1-GFP to the LD by α-particle microbeam irradiation in stably 53BP1-GFP–expressing C2C7 MB (blue curve) in growth medium, MC (black curve) in differentiation medium (DM), and MT (red curve) in DM. The irradiation was applied at t = 10 s. N ≥ 3 independent experiments with 26–100 nuclei/cell type, mean ± SEM.