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. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure S2. Transcriptional activity during myofibrillogenesis.

Figure S2.

(A) Images of 5-Ethynyl Uridine (EU) incorporation measuring mRNA production for each condition during differentiation, in myoblasts (MB) that were isolated from muscles of 5 d old mice, myoblasts that were induced to fuse (MBF), myotubes of 4 d (MT4D), and myotubes of 7 d (MT7D). DAPI signal is depicted in blue and EU incorporation is illustrated in red. Nuclei are delimited by dashed lines. Scale bar, 5 µm. (B) Quantification of RNA synthesis determined by EU incorporation in the course of myogenic differentiation, in MB, MBF, MT4D, and MT7D. Error bars represent the SEM obtained from at least 50 cells and data are representative of three independent experiments, significance by t test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. (C) Schematic representation of rDNA unit and localization of the 47S pre-rRNA probe. (D) Images of 47S RNA-FISH for each condition during differentiation, in MB, MBF, MT4D, and MT7D. DAPI signal is in blue and 47S probe is illustrated in red. Nuclei and nucleoli are delimited by dashed and dotted lines, respectively. Scale bar, 5 μm. (E) Quantification of 47S Synthesis determined by 47S probe hybridization in the course of myogenic differentiation, in MB, MBF, MT4D, and MT7D. Error bars represent the SEM obtained from at least 50 cells and data are representative of three independent experiments, significance by t test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.