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. 2023 Nov 22;7(2):e202302279. doi: 10.26508/lsa.202302279

Figure S8. Disappearance of double-strand break markers upon X-ray irradiation.

Figure S8.

(A) Representative images of stably 53BP1-GFP–expressing C2C7 myoblasts at the indicated time after 5 Gy of X-ray irradiation immunolabelled with antibodies against double-strand break markers: 53BP1 (magenta) γH2AX (red). DNA was stained with DAPI (grey). Scale bars, 10 μm. (B, C) Quantification of 53BP1 foci (B) and γH2AX foci (C) per nucleus in C2C7 myoblasts, un-transfected (blue curve) or stably transfected with 53BP1-GFP–expressing plasmid (dashed black curve) at the indicated time upon 5 Gy of X-ray irradiation. N = 1 experiment with 20–60 nuclei/cell type, mean ± SEM for each condition.