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. 1983 Nov;73(3):858–859. doi: 10.1104/pp.73.3.858

Isolation, Purification, and Partial Characterization of Formate Dehydrogenase from Soybean Seed 1

Michael P Farinelli 1, David W Fry 1, Keith E Richardson 1
PMCID: PMC1066562  PMID: 16663314

Abstract

Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4°C, and has Km values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively.

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Selected References

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