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. 2023 Nov 7;98:104865. doi: 10.1016/j.ebiom.2023.104865

Fig. 8.

Fig. 8

Blockade of IL-6R prevents IL-1α-induced microbiome alterations in the neonatal caecum and colon. Samples from the neonatal colon and caecum, as well as environmental controls, were obtained at postnatal day 21 using sterile swabs. (a–f) Principal coordinate analysis (PCoA) illustrating variation in the metagenomic profiles of the neonatal caecum of the (a) PBS/Isotype and IL-1α/Isotype groups or (d) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). Bar plots showing the taxonomic classifications of the 20 bacterial taxa with highest relative abundance in the neonatal caecum of the (b) PBS/Isotype and IL-1α/Isotype groups or (e) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). Heatmap displaying the relative abundance and taxonomy of the 20 most relatively abundant bacterial taxa between the metagenomic profiles of the neonatal caecum of the (c) PBS/Isotype and IL-1α/Isotype groups or (f) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). (g–l) PCoA illustrating variation in the metagenomic profiles of the neonatal colon of the (g) PBS/Isotype and IL-1α/Isotype groups or (j) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). Bar plots showing the taxonomic classifications of the 20 bacterial taxa with highest relative abundance in the neonatal colon of the (h) PBS/Isotype and IL-1α groups/Isotype or (k) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). Heatmap displaying the relative abundance and taxonomy of the 20 most relatively abundant bacterial taxa between the metagenomic profiles of the neonatal colon of the (i) PBS/Isotype and IL-1α/Isotype groups or (l) PBS/aIL-6R and IL-1α/aIL-6R groups (n = 10–12 per group). Similarities in the metagenomic profiles (PCoA plots) were characterised using the Bray–Curtis similarity index. For bar plots, multiple taxa with the same phylum-level classification within the same sample are indicated by bars of the same colour. For heatmaps, bacterial taxa with significantly altered abundance between groups are labelled in blue. p-values were determined using two-sided Mann–Whitney U-tests with Holm's correction for multiple comparisons.