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. 2023 Nov 2;78:101831. doi: 10.1016/j.molmet.2023.101831

Figure 6.

Figure 6

Post activation recycling of GIPR-Q354 is impaired in β−cells. MIN6 cells were electroporated with HA-GIPR-E354-GFP or HA-GIPR-Q354-GFP A. Quantification of GIPR plasma membrane (PM) level to total distribution in basal and GIP-stimulated (100 nM) cells for 1 h. Data from individual experiments are normalized to the HA-GIPR-E354-GFP-electroporated cells in basal condition (n = 11 independent experiments). B. Cells were stimulated or not with GIP (100 nM) for 1 h. Immunofluorescence shows HA-GIPR-E354-GFP or HA-GIPR-Q354-GFP (green), TGN46 (magenta) and nuclei counterstained with DAPI (blue). Scale bar, 10 μm. C. Cells were stimulated or not with GIP (100 nM) for 1 h prior to incubation with anti-HA antibodies for the indicated times. Graph shows GIPR exocytosis rate in basal or GIP-stimulated cells (n = 4 independent experiments). D. Cells were stimulated with GIP (100 nM) for 1 h followed by an up to 2 h washout. Graph shows quantification of GIPR plasma membrane (PM) level to total distribution at different time points (n = 5 independent experiments). Data are mean ± SEM. One tailed paired t-tests for A, D. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)