Video 1. Growth of a typical low-passage human Schwann cell (hSC) culture.

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A single cell suspension of adult hSCs (proliferative) obtained by trypsin dissociation was plated on a laminin-coated dish and incubated in HP medium up until confluency, as described in Protocol 1A. Notice the fast adhesion of the cells, the changes in cell morphology (including process extension and alignment), the rapid cell migration, and the asynchronous appearance of mitotic figures. This culture can be considered confluent and ready to use by the third day of culture. The cells were imaged using IncuCyte ZOOM^TM using 20× objective lenses. Individual images were taken every 20 min for a total time of 84 h from the onset of plating. Some relevant elements were highlighted in this and other videos.