Video 5. Characteristics of magnetic-activated cell sorting (MACS)-purified fibroblast cultures.

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The MACS-purified human fibroblasts (eluted fraction) were obtained from the cell cultures shown in Video 3. The cells were plated immediately after purification. Notice that the fibroblasts are highly proliferative, and their phenotype is clearly distinguishable from that of hSCs. Most fibroblasts exhibit a flat and expanded morphology with a conspicuous reticulated cytoplasm at confluency.