Fig. 5.

Dietary SeNPs supplementation activated the AMPK/Nrf2/NLRP3 signaling pathway of early-weaned piglets. (A) The expression levels of AMPK and pAMPK proteins were measured by Western blot analysis. (B) The expression levels of NLRP3 inflammatory proteins were detected by Western blot analysis. (C) Nrf2 activation and the expression levels of its downstream proteins were measured by Western blot analysis. n = 4. SeNPs = selenium nanoparticles; AMPK = AMP-activated kinase; pAMPK = phospho-AMPK; NLRP3 = NOD-like receptor thermal protein domain associated protein 3; ASC = apoptosis-associated speck-like protein containing a caspase recruitment domain; IL-1β = interleukin-1β; IL-18 = interleukin-18; Nrf2 = nuclear factor erythroid 2-related factor 2; NQO-1 = NADPH dehydrogenase-1; HO-1 = heme oxygenase-1.