Figure 2.
GBP1 expression modulates tumor progression and ascities formation in the ID8 intraperitoneal ovarian cancer mouse model
1 x 106 ID8 cells stably transduced with GBP2b overexpression (OV) or vector control (VC) or scrambled control (SC) or GBP2b shRNA (KD) were implanted intraperitoneally in 9- to 10-weeks-old female C57BL/6 mice.
(A) Left: representative image of VC and GBP2b-OV mice after 40 days of IP implantation. Right: Kaplan-Meier plot for the survival of VC and OV ID8-implanted mice (n = 10/group).
(B) Left: representative images of SC and GBP2b-KD mice after 40 days of IP implantation. Right: Kaplan-Meier plot for the survival of SC and KD ID8-implanted mice (n = 10/group). Significantly different compared with respective controls (VC or SC) by Log rank (Mantel-Cox) test.
(C and D) Ascites fluid was collected from each mouse after 40 days of IP implantation using an 18G needle. Total fluid volume was measured using 10 mL serological pipette (n = 10/group).
(E and F) The body weight of each mouse was measured before ascites fluid collection. Data are shown as mean ± SD. Significantly different compared with respective controls by Student’s t test.