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. 2023 Oct 24;26(11):108292. doi: 10.1016/j.isci.2023.108292

Figure 5.

Figure 5

GBP1 modulates proteasomal activity

(A) Immunoblotting of total ubiquitinated proteins in OC cells stably transduced with GBP1 (GBP2b in ID8) overexpression (OV) or vector control (VC) or scrambled control (SC) or GBP1 shRNA (GBP2b in ID8) (KD).

(B) Densitometry analysis of three independent immunoblotting of total ubiquitinated proteins in OC cells stably transduced cells. Data are shown as mean ± SD of triplicate samples. Significant differences compared to respective controls were assessed by Student’s t test.

(C) Proteasomal activity assessment of transduced OC cells. An equal amount of protein from each cell line was analyzed for its proteasomal activity using a proteasome substrate (Succ-LLVY-AMC) utilization-based assay.