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. 2023 Nov 9;14:1205834. doi: 10.3389/fimmu.2023.1205834

Figure 8.

Figure 8

A novel anti-inflammatory role of dapagliflozin in macrophages independent of glucose concentrations in vitro. (A) QPCR analysis showing Csf2rb, Btla, and Isg15 mRNA levels in RAW 264.7 cells cultured in normal-glucose (Ctrl), high-glucose (Case), and high-glucose with dapagliflozin (Dapa) medium. (B) Western blot analysis showing CSF2RB, BTLA, and ISG15 protein levels in macrophages of Ctrl, Case, and Dapa groups, with corresponding quantifications in (C). (D) Scatter plot indicating distinct protein expression levels of macrophages in Ctrl versus Case group. (E) GO enrichment analysis suggesting endothelial cell apoptosis and leukocyte chemotaxis pathways in macrophages in the high-glucose medium. (F) Scatter plot indicating distinct protein expression levels of macrophages in Case versus Dapa group. (G) QPCR analysis showing Tnf-α, Il-1β, iNos, Il-10, and Tgf-β mRNA levels in macrophages of Ctrl, Case, and Dapa group. NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA test.