Fig. 2.

Hepatic mitochondrial dysfunction and ROS production were ameliorated in SHP-null mice fed WD. A: Representative electron micrographs of WT and Shp^−/− mice livers are depicted. Arrow points to mitochondrium, whose image is magnified in an inset. The scale bar represents 1 μm. B: Expression of mitochondrial-encoded genes from livers of WT and Shp^−/− mice fed control diet (CD) or WD (left panel). Mitochondrial-specific citrate synthase activities were quantified from liver extract as described in the Methods section (right panel) (n = 5–6). C: relative mitochondrial DNA content was presented by PCR amplification units of mitochondrial-specific 12 rRNA genes after normalization with nuclear-specific globin gene amplification (n = 6). D: hepatic ROS production was presented by fluorescence intensity per wet liver weight after dihydroethidium injection as described in the Materials and methods section (n = 4–7). E: hepatic expression of NADPH oxidase genes in WT and Shp^−/− mice (n = 5–6). Cox1, cytochrome c oxidase subunit 1; Ctyb, cytochrome b; LD, lipid droplet; ND2, NADH dehydrogenase subunit 2; Nu, nucleus.