Fig. 5.

Hepatic lipid and inflammatory gene expression were assessed with WT and Shp^−/− mice after 1-month MCD diet regimen. The 2-month-old experimental mice were fed amino acid-defined control diet (Con) or MCD diet for 1 month. Livers and blood were collected at the end of regimen after overnight fasting. A: Hepatic TG and cholesterol levels were presented after normalization by wet liver weight. B: Plasma levels of TG and cholesterol from the mice. Expression of hepatic genes involved in inflammation (C), anti-inflammation (D), and M2 macrophage markers (E) was presented as means ± SEM (n = 5). Two-way ANOVA results were shown with P values. Gen, genotype; Int, interaction.