TABLE 1.
Fluorogenic substrates used to detect enzymatic activities of actinomycete spores with a spectrofluorometer with the indicated wavelengths
| Fluorogenic substratea | Enzymes detected with substrate | Fluorescence measurement
|
|
|---|---|---|---|
| Excitation wavelength (nm) | Emission wavelength (nm) | ||
| MUA | Esterases | 320 | 450 |
| MUB | Lipases | 320 | 450 |
| MUPr | Lipases | 320 | 450 |
| FDA | Esterases | 480 | 515 |
| FDPr | Lipases | 480 | 515 |
| RSZ | Dehydrogenases | 560 | 590 |
| MUPh | Phosphatases | 320 | 450 |
a MUA, MUB, MUPr, and MUPh were from Sigma Chemical Co., St. Louis, Mo.; FDA, FDPr, and RSZ were from ICN Biochemicals Inc., Aurora, Ohio.