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. 2023 Nov 15;120(47):e2307551120. doi: 10.1073/pnas.2307551120

Fig. 2.

Fig. 2.

TMEM16A transiently regulates ASL pH under inflammatory conditions. (A) Schematic overview of the study. (B) Summary ASL pH traces. Solid black trace CF-CRISPRCas9 CTRL (CF) and dotted black trace CF-TMEM16AKO epithelia (CF-T16AKO) under normal conditions. (C) Summary ASL pH traces. Solid red trace CF-CRISPRCas9 CTRL (CF) and dotted red trace CF-TMEM16AKO (CF-T16AKO) under inflammatory conditions (IL-4, 10 ng/mL for 48 h). Baseline ASL pH was measured by adding carbachol (CCh, 50 µM), and forskolin (FSK, 10 µM) as indicated. (D) Baseline ASL pH. ASL pH was calculated as the average of five points before the addition of CCh. (n = 45, three donors, 2-way ANOVA with Tukey's multiple comparisons test). (E) CCh ASL pH. ASL pH was calculated as the average of five points after the addition of CCh. (n = 27, three donors, 2-way ANOVA with Tukey's multiple comparisons test). (F) FSK ASL pH. ASL pH was calculated as the average of five points 2 h after the addition of FSK. (n = 27, three donors, 2-way ANOVA with Tukey's multiple comparisons test). Data are presented as mean ± SD; *P < 0.05, **P < 0.01, and ****P < 0.0001.