Fig. 3.

Sustained increases in cytosolic calcium raise ASL pH in CF nasal epithelia under inflammatory conditions by activating TMEM16A. (A) Summary ASL pH traces. Solid black trace CF-CRISPRCas9 CTRL (CF) and dotted black trace CF-TMEM16AKO epithelia (CF-T16AKO) under normal conditions. (B) Solid red trace CF-CRISPRCas9 CTRL (CF) and dotted red trace CF-TMEM16AKO (CF-T16AKO) under inflammatory conditions (IL-4, 10 ng/mL for 48 h). Baseline ASL pH was measured followed by the addition of CPA (10 µM) followed by carbachol (CCh, 50 µM), and then forskolin (FSK, 10 µM) as indicated. (C) CPA ASL pH. ASL pH was calculated as the average of five points 2 h after the addition of CPA. (n = 9, three donors, 2-way ANOVA with Tukey's multiple comparisons test). Data are presented as mean ± SD; *P < 0.05.