Fig. 4.

SLC26A4 regulates both baseline and forskolin-stimulated ASL pH under inflammatory conditions. (A) Summary ASL pH traces. Left, solid black trace CF-CRISPRCas9 CTRL (CF) and dotted black trace CF-SLC26A4KO epithelia (CF-26A4KO) under normal conditions. (B) Solid red trace CF-CRISPRCas9 CTRL (CF) and dotted red trace CF-SLC26A4KO (CF-26A4KO) under inflammatory conditions (IL-4, 10 ng/mL for 48 h). Baseline ASL pH was measured followed by the addition of carbachol (CCh, 50 µM), and then forskolin (FSK, 10 µM) as indicated. (C) Baseline ASL pH. ASL pH was calculated as an average of five points before the addition of CCh. (n = 12, three donors, 2-way ANOVA with Tukey’s multiple comparisons test). (D) FSK ASL pH. ASL pH was calculated as an average of five points after 2 h of the addition of FSK. (n = 12, three donors, 2-way ANOVA with Tukey's multiple comparisons test). Data are presented as mean ± SD; **P < 0.01 and ****P < 0.0001.