Fig. 5.

FDA-approved drugs, alprostadil and indacaterol maleate, alkalinized ASL pH under inflammatory conditions by activating SLC26A4. (A) Summary ASL pH traces. Solid blue trace CF-CRISPRCas9 CTRL (CF) and dotted blue trace CF-SLC26A4KO epithelia (CF-26A4KO) under normal conditions. (B) Summary ASL pH traces. Solid green trace CF-CRISPRCas9 CTRL (CF) and dotted green trace CF-SLC26A4KO (CF-26A4KO) under inflammatory conditions (IL-4, 10 ng/mL for 48 h). Baseline ASL pH was measured followed by the addition of alprostadil (Left) or indacaterol maleate (Right–both 3 µM). This was followed by carbachol (CCh, 50 µM) and then forskolin (FSK, 10 µM) as indicated. (C) ASL pH was calculated as an average of five points 2 h after the addition of alprostadil and indacaterol maleate. (n = 7, three donors, 2-way ANOVA with Tukey's multiple comparisons test). (D) FSK ASL pH. ASL pH was calculated as an average of five points after 2 h of the addition of FSK. (n = 7, three donors, 2-way ANOVA with Tukey’s multiple comparisons test). Data are presented as mean ± SD; *P < 0.05 and ****P < 0.0001.