Figure 4.

Neutralization assays: Neutralizing effect on PsV infection by different drugs (a) Linaclotide, (b) Actinomycin, (c) Dihydroergotamine, and (d) Mithramycin at different concentrations (1.56, 3.1, 6.2, 12.5, 25, 50 uM). The 293T and 293T-ACE2 cells were used as a negative control without drug (Control and ACE2) and with drug (XControl, XACE2), respectively. The infection of PsV were evaluated in 293T cells (PsV Control) as a nonincorporation control and in 293T-ACE2 cells as a positive control (PsVACE2). The luminescence exposition was measured after 72 h of treatment. All data are shown as mean ± SD. For more details about p-values see Table S1 in the Supporting Information. The values in the table indicate p < 0.001 statistically significant data analyzed by two-way ANOVA.