Figure 5.

(A) TNF-α, (B) IL-6, and (C) IL-1β levels in the medium were determined using an ELISA kit; values are mean ± SEM of three independent experiments. The inhibition of (D) Akt and (E) JNK phosphorylation protein expressed by agarperoxinol B on LPS-activated microglial cells. Protein levels were determined by using western blot analysis. The relative intensity of p-Akt/Akt and p-JNK/JNK bands were calculated by densitometry. The data were presented as mean ± SEM (n = 5). ^#p < 0.0001 was compared with the sample of the control group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 were compared with the LPS group.