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In vitro anticancer effects of the DOX loaded N–P hydrogel by transwell system: (a) cell viability rate of B16–F10 melanoma cell by CCK (n = 5, **p < 0.01; ***p < 0.001), and (b) Fluorescence image of 2 and 24 h (scale bar = 150 μm, Endosome/lysosome and nuclei were stained with Lysotracker Green DND-26 and DAPI, respectively).
