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. 2023 Nov 23;11:185. doi: 10.1186/s40478-023-01687-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Representative images of RNAscope combined with immunofluorescence in Lewy body disease cases. A A neuron without α-synuclein (α-syn) immunoreactivity (IR) in the substantia nigra, which has numerous SNCA and neuron-specific RBFOX3 transcripts in the nucleus and cytoplasm. B Punctate α-syn IR (arrow) in a neuron, which shows many fine dots-like α-syn IR, and an irregular-shaped compact inclusion (arrowhead), which shows compact α-syn IR without a core or halo. Numerous SNCA transcripts are observed in the neuron containing the punctate α-syn IR compared with the neuron with irregular-shaped compact inclusion. C Brainstem-type Lewy body (bLB) containing a round core and pale halo shows few SNCA transcripts. D. A neuron containing a punctate α-syn IR in the amygdala shows numerous SNCA transcripts. E. A neuron without α-syn IR (arrow) and a cortical LB (cLB, arrowhead) in the amygdala show few SNCA transcripts. F. Astrocytes with (arrowhead) and without (arrows) α-syn IR in the amygdala contain few SNCA transcripts. Each image set represents confocal images taken from the same field of view showing phosphorylated-α-syn (p-Syn, A–E) or 5G4 α-syn (5G4-syn, F) immunostaining (green), SNCA (red), neuron-specific RBFOX3 (A–E) or astrocyte-specific ALDH1L1 (F, magenta), DAPI (blue), and the enlarged merged image. Scale bars represent 20 μm