Extended Data Fig. 10. Overexpression of mouse or nmrHAS2 protects cells from oxidative stress.

a. Pulse field gel shows that mouse skin fibroblasts (MSF) overexpressing mouse HAS2 (mHAS2) or nmrHAS2 produce more hyaluronic acid compared to fibroblasts transfected with empty vector (EV). HAase-treated samples were run in parallel to confirm the specificity of HA staining. Media from three different cell lines was pooled for HA extraction.

b. HA ELISA shows that mouse skin fibroblasts (MSF) overexpressing mHAS2 or nmrHAS2 produce more hyaluronic acid compared to fibroblasts transfected with empty vector (EV). p-values were calculated using unpaired two-tailed t-test, bars represent the means, error bars show standard errors, dots represent technical replicates.

c. Mouse skin fibroblasts overexpressing mHAS2 or nmrHAS2 are more resistant to H₂O₂ treatment. p-values were calculated using unpaired two-tailed t-test, error bars show standard errors, dots represent technical replicates.