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Temperature-dependent changes in calculated α-helical contents. (A) A. fumigatus phytase. (B) A. niger T213 phytase. (C) A. niger pH 2.5 acid phosphatase. The α-helical contents were calculated from the CD spectra shown in Fig. 2. Symbols: •, α-helical contents after 20 min of incubation at 30, 50, 70, 80, or 90°C; ○, α-helical contents after a subsequent 1-h renaturation period at 30°C.
