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Temperature-dependent enzyme inactivation. (A) A. fumigatus phytase. (B) A. niger T213 phytase. (C) A. niger pH 2.5 acid phosphatase. The individual acid phosphatases were incubated for 20 min at 30, 50, 70, 80, or 90°C and then immediately put on ice. The results represent means ± standard deviations (n = 3). Enzymatic activities were measured as described in Materials and Methods.
