FIG. 3.

Time course for ¹⁴CO₂ formation from Pyr-¹⁴CN and Kg-¹⁴CN catalyzed by cell extracts from cyanide-induced cells of P. fluorescens NCIMB 11764. Reaction mixtures contained 4 mM NADH where indicated below and 2 mM (1 to 2 μCi) substrate. ■ and □, Pyr-¹⁴CN under aerobic (with NADH) and anaerobic (without NADH) conditions, respectively; ⧫ and ◊, Kg-¹⁴CN under aerobic (with NADH) and anaerobic (without NADH) conditions, respectively. Each value is the average of results from three separate experiments. Standard errors ranged from 5 to 15% but are not shown for clarity.