TABLE 4.
Sustained ability of isolate RB1 to grow on unamended seawater
| Incubation time (days) | Population (cells/ml) | Dry mass (fg/cell) | Vol (μm3/cell)a | Vol of ln cells (μm3) | Avg DNA content (fg/cell) |
|---|---|---|---|---|---|
| 0b | 0.05 | 37.8 | 0.18 | 0.10 | 4.1 |
| 18 | 30,000 | 41.1 | 0.20 | 0.17 | 4.4 |
| 0c | 3,000 | 41.1 | 0.20 | 0.17 | 4.4 |
| 21 | 180,000 | 48.9 | 0.23 | 0.19 | 4.8 |
a Calculated from light scatter intensity, a dry weight/wet weight ratio of 0.20, and a density of 1.04 g/cm3.
b The inoculum was subcultured 28 times on 100 mg of acetate liter−1, diluted to 50 cells in 5 μl, and added to 50 ml of unamended seawater.
c The inoculum was a 1:10 dilution of the 18-day culture.