TABLE 5.

Effect of experimental protocol on the kinetic constants for toluene uptake

Biomass wet wt (ng/ml)	Incuba-tion time (min)	a°QB (liters/mg of cells/h)a	KA (μg/liter)b	Km (μg/liter)	Vmax (μg of B/mg of cells/h)c	a°B (liters/ mg of cells/hc)
2,590	4	0.55	1.3	0.9	>2	2.2
600d	4.5	2.7				10.6
2.4	90	12b	34	60	>1,215	47.4

^a Partial affinity calculated from that portion of toluene (substrate B) used and collected as product, P, or carbon dioxide, Q. 

^b Concentration at half-maximal specific affinity. 

^c Calculated from carbon dioxide production and yield. 

^d Uptake using toluene-limited cells grown in continuous culture.