FIG. 2.

Sequence alignment of β-tubulin sequences from 14 C. parvum isolates. The alignment was generated with the PileUp program (Wisconsin Sequence Analysis Package). Isolate codes are shown on the left. The number following the hyphen designates the plasmid number. The multilocus RFLP genotype (15) is indicated leftmost. Only the polymorphic positions are shown. Changes from the sequence under GenBank accession no. Y12615 are shaded. A 6-bp duplication in Y12615 presumed to be artifactual was omitted (see text). Selected positions are numbered below the sequences according to accession no. Y12615. The intron-exon boundary is indicated with a vertical line. The arrowhead at position 399 indicates the 5′-most nucleotide of a polymorphic Tsp509I site in exon 2. Clones icp-1, tamu-2, tamu-5, 0541L-5, 0541L-6, 0541L-8, 2066K-5, and 0583K-8 were sequenced on both strands. All others were sequenced on one strand. A sequence ambiguity in 0541L-9 is indicated in lowercase.