Fig. 1.

Schematic diagram highlighting the key steps of PCTS production from human tumour explants. The timeline illustrated in the final row shows: the overall culture timeline (8 days) (top) with the corresponding drug screening experiment timeline (bottom), the collection timepoints indicate the duration of drug exposure. In drug screening experiments, slice treatment starts after 1 day in culture (indicated in green). All subsequent graphs representing longitudinal data are labelled “Time in culture” or “Time post treatment” in concordance with this schematic.