Fig. 6.
Effect of multi-kinase inhibitor, checkpoint inhibitor (anti-PD1) and combination therapy on PCTS from patient 168: tumour slices had reduced cell proliferation in response to immunotherapy within 8 days of culture. Tissue slices from pt 168 were treated with doxorubicin (red), anti-PD-1 blocking antibody (blue), regorafenib (pink) and combination therapy (purple) and compared to untreated control (black). The following parameters were assessed in nPCTS ≥2 per timepoint. a | Cell proliferation, shown as percentage of Ki67 positive cells, in PCTS treated with the indicated drugs at the indicated time points; mean ± SD. b | Apoptosis, shown as percentage of TUNEL positive cells, in PCTS treated with the indicated drugs at the indicated timepoints; mean ± SD. Statistical test: 2-way ANOVA.