Fig. 7.
Effect of multi-kinase inhibitor, checkpoint inhibitor (anti-PD1) and combination therapy on PCTS from patient 168: tumour slices respond to immunotherapy within 8 days of culture. Tissue slices from pt 168 were treated with doxorubicin (red), anti-PD-1 blocking antibody (blue), regorafenib (pink) and combination therapy (purple) and compared to untreated control (black). The following parameters were assessed in nPCTS ≥2 per timepoint. a | Weight-adjusted intracellular ATP levels of tissue slices. b | AEC of tissue slices from patient 168 treated with indicated treatment combinations. c | Representative images for H&E, haematoxylin + Sirius red, Ki67, TUNEL, CD45 (leucocytes) and CD3 (T-cells) for treated tissue slices. Scale bars: 100 μm, unless otherwise indicated.