Figure 2.

Physical activity of SAAR mice is minimally influenced by sex. (a) Middle-aged mice were defined as 1-year-old male and female mice and were fed either a control (CF, 0.86% met w/w, white bars or circles) or SAAR (0.12 met w/w, black bars or circles) diet for 52 weeks. White dots within bar graphs indicate values from each animal. Muscle mass (b) was obtained from the quadriceps femoris. Physical activity was assessed by wire hang and shown as holding impulse (c) and accelerated rotarod (d) performed 2 weeks prior to sacrifice. Distance run (e and f) and speed (f and g) from 24-h in-cage voluntary running wheel measurements collected every 2 weeks after 6 months on the diet. Statistical analysis was conducted using 2-way ANOVA with Sidak post hoc test, as described in methods (n = 7–8 per group, ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001).