Figure 3.

The anticancer activities of the three pure compounds (1–3) isolated from Ziziphus spina-christi using the MTS assay on glioblastoma, breast cancer, lung, and colorectal carcinoma cells. Cancer cells lines A549 (a) U87 (b), MDA-MB-231 (c) and CT-26 (b) were tested. Cells (2 × 10⁴ cells per well in 96 well plates) were treated with pure compounds as using the following concentrations (1, 5, 10 or 20 μg/mL) or a matching solvent control for 24 h at 37 °C with 5% CO₂. Cells were stained with MTS reagents and cell survival was quantitated using the spectramax plate reader. The graphs represent the analysis of six replicates and show the percentage of survival of A549, U87, MDA-MB-231 and CT-26–- treated cells relative to matching solvent-treated cells. The solvent used is methanol. All experiments were performed at least two times. *p < 0.05, Student’s t-test.