Table 4. The fitting output was reproduced independently by other users.
The compilation of fitting results generated by two independent users for AE.A244 binding to CH31 data on all three platforms is shown. SE is abbreviation for standard error.
| Carterra LSA Replicates | Biacore T200 | Octet RED384 | |||||
|---|---|---|---|---|---|---|---|
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | ||||
| User 1 | k a (M -1 s -1) | 4.07E+03 | 5.50E+03 | 2.61E+03 | 3.71E+03 | 1.81E+03 | 5.68E+03 |
| SE k a (M -1 s -1) | 1.18E+02 | 1.11E+02 | 9.03E+01 | 9.07E+01 | 8.73E+00 | 7.50E+01 | |
| k d (s -1) | 5.14E-05 | 7.78E-05 | 5.53E-05 | 5.99E-05 | 1.19E-04 | 2.94E-04 | |
| SE k d (s -1) | 1.37E-06 | 1.38E-06 | 1.13E-06 | 1.14E-06 | 8.04E-07 | 4.59E-06 | |
| K D (M) | 1.26E-08 | 1.41E-08 | 2.12E-08 | 1.61E-08 | 6.57E-08 | 5.17E-08 | |
| SE K D (M) | 4.97E-10 | 3.79E-10 | 8.52E-10 | 4.99E-10 | 5.45E-10 | 1.06E-09 | |
| User 2 | k a (M -1 s -1) | 4.07E+03 | 5.50E+03 | 2.61E+03 | 3.71E+03 | 1.81E+03 | 5.68E+03 |
| SE k a (M -1 s -1) | 1.18E+02 | 1.11E+02 | 9.03E+01 | 9.07E+01 | 8.73E+00 | 7.50E+01 | |
| k d (s -1) | 5.14E-05 | 7.78E-05 | 5.53E-05 | 5.99E-05 | 1.19E-04 | 2.94E-04 | |
| SE k d (s -1) | 1.37E-06 | 1.38E-06 | 1.13E-06 | 1.14E-06 | 8.04E-07 | 4.59E-06 | |
| K D (M) | 1.26E-08 | 1.41E-08 | 2.12E-08 | 1.61E-08 | 6.57E-08 | 5.17E-08 | |
| SE K D (M) | 4.97E-10 | 3.79E-10 | 8.52E-10 | 4.99E-10 | 5.45E-10 | 1.06E-09 | |