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. 2023 Nov 9;3(11):100364. doi: 10.1016/j.xgen.2023.100364

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

SCRaMbLE of YSy142 disomic yeast

(A) SCRaMbLE and analysis workflow. The auxotrophic marker of the YSy142 strain was swapped from HIS3 to MET15. See construction of aneuploid synVII strain section in the STAR Methods for details.

(B) The fate of each segment flanked by two loxPsym sites in each strain is indicated as preserved (light blue) or deleted (white) by any SCRaMbLE event. The y axis shows the relative average phenotypic recovery rate of each SCRaMbLEd strain in comparison with that of YSy142 represented by the color scale (n ≥ 200).

(C) The distribution of recombination events for all selected SCRaMbLEd aneuploid strains. The number of events per strain has a long tail with some strains having 22 and 25 distinct recombination events.

(D) Correlation between chromosome retention rate and growth recovery in SCRaMbLEd strains with circular synVII and SCRaMbLEd strains with linear synVII. Each dot represents one SCRaMbLEd strain. R indicates Pearson’s correlation. Solid line: fitted curve (ggplot:geom_point), geom_smooth (method = lm, se = TRUE). Gray area: 95% confidence range for fitted curve.