Figure 4.

Growth assay and ESR profiling of disomic yeast with circular SCRaMbLEd synVII

(A) General improvement of fitness at varying degrees was observed for the top 18 SCRaMbLEd strains in five representative conditions. Each dot represents average growth recovery rate of one SCRaMbLEd strain calculated based on multiple single colonies (n ≥ 200).

(B) Spot assay of two representative strains of the 18 SCRaMbLEd strains under various conditions, showing general improved fitness.

(C) The ESR genes expression profile of the unSCRaMbLE strain YSy142 and two SCRaMbLEd strains (YSy151 and YSy150) after normalization with the reference wild-type strain BY4741 in three selected conditions. The fold change is represented by the color scale (yellow: upregulated; blue: downregulated). Conditions include: YPD at 30°C for 2 days; YPD + cycloheximide (Cyc; 0.01 μg/mL) at 30°C for 2 days; YPD + dl-dithiothreitol (DTT; 2.5 mM pretreatment for 1 h) for 2 days; YPD + hydroxyurea (HU; 100 mM) at 30°C for 4 days; YPD + methyl methanesulfonate (MMS; 0.01% v/v) at 30°C for 3 days.

YPD, yeast extract peptone dextrose.