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. 2023 Nov 9;3(11):100379. doi: 10.1016/j.xgen.2023.100379

Figure 3.

Figure 3

Synthetic DNA regions of chromosome XIV in haploid progeny of a synXIV-BY4741 meiotic cross

(A and B) Slow- (A) and fast-growing (B) strains were tested for their synthetic DNA content using PCR tag analysis. Megachunk regions A–X of chromosome XIV are displayed for each strain, with blue representing synthetic DNA and white wild-type DNA. All strains are haploid derivates of a cross between strains 1 and 40 (Table S1).

(C) Serial 10-fold dilutions of wild-type (BY4741), synXIV (strain 70; Table S1), and SynXIV with the IRA1A1259D mutation reverted to wild type (strain 71; Table S1). Each strain was plated on YP glycerol (YPG) at 30°C and 37°C for 4 days prior to imaging.